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KMID : 1094020160330060346
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Journal of Veterinary Clinics
2016 Volume.33 No. 6 p.346 ~ p.350
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PCR-based Detection of Bovine Papillomavirus DNA from the Cutaneous Papillomas and Surrounding Environments in the Korean Native Cattle, Hanwoo
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Han Sang-Hyun
Park Yong-Sang
Seo Jong-Pil
Kang Tae-Young
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Abstract
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Two 1-year old calves of Korean Native cattle (Hanwoo) presented cutaneous papillomas on the face andneck. Type 2 bovine papillomavirus (BPV-2) was identified in the cutaneous papillomas based on BPV-specific PCRand subsequent DNA sequencing analysis results. Using DNA samples extracted from two affected calves and unaffectedanimals reared in the same stable, BPV-2 was not only detected in the cutaneous papillomas of affected animals basedon BPV-specific PCR analysis, but also detected in normal skins, hairs, and their environments based on nested PCRanalysis. BPV-2 was also detected in DNA samples isolated from animals and environments of that distinct stablewith affected calves. However, no BPV-2 was detected in the drinking water of both stables (infected and unaffected). These findings concluded that BPV-2 was transmitted by direct or indirect contact, not by drinking water. This isthe first report to show molecular evidence of BPV-2 infection. Rapid and precise molecular identification can beused to screen BPV-2 in cattle farms to understand the biological roles of BPV in animal diseases.
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KEYWORD
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bovine papillomavirus, polymerase chain reaction, BPV-2
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